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 Directed Evolution English | 2022 | ISBN: 1071621513 | 280 Pages | PDF (True) | 7 MB This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Milakovich, "Digital Governance Ed 2" English | ISBN: 1032104910 | 2021 | 474 pages | EPUB | 2 MB The application of digital information and communication technologies (ICTs) to reform governmental structures and public service is widely and perhaps naively viewed as the 21st century "savior", the enlightened way to reinvigorate democracy, reduce costs, and improve the quality of public services.  Digital Business in Africa: Social Media and Related Technologies English | 2022 | ISBN: 3030934985 | 415 Pages | PDF EPUB (True) | 6 MB This edited volume seeks to examine how enterprises in Africa can utilize digital technologies and innovations in creating value for their customers and clients in order to increase effectiveness and efficiency. Through social media, businesses are increasingly reaching and engaging their customers in several ways and so enterprises in Africa must harness the opportunities in the digital space if they want to remain competitive, earn profit, and meet their customers' needs. Accordingly, this book looks at how digital technologies are helping shape the financial, educational, and advertising sectors in Africa. As digital technologies raise challenges, the chapters that follow will discuss ethical and social practical frameworks to effective digital business in Africa. This volume promises to fore both theoretical underpinnings, and practical implementations of digital technologies in the African business context.  Digital Business and Intelligent Systems: 15th International Baltic Conference, Baltic DB&IS 2022, Riga, Latvia, July 4-6, 2022, Proceedings by Mirjana Ivanovic English | PDF | 2022 | 273 Pages | ISBN : 3031098498 | 21.1 MB This book constitutes the refereed proceedings of the 15th International Baltic Conference on Digital Business and Intelligent Systems, Baltic DB&IS 2022, held in Riga, Latvia, in July 2022.  Differential Equations by Leila Spark English | 2022 | ISBN: N/A | ASIN: B0B5NF5CBH | 408 pages | EPUB | 3.56 Mb Differential Equations is a fully reference book for students of high (Grades 9-12), mathematics, physics, engineering and diploma courses.  Differential Diagnosis of Movement Disorders in Clinical Practice By Abdul Qayyum Rana, Peter Hedera (auth.) 2014 | 112 Pages | ISBN: 3319016067 | PDF | 1 MB This practical, pocket-sized, quick reference book is for those who need to manage movement disorder patients without the immediate help of a movement disorder neurologist. Only the most pertinent information is covered.This book is divided into five chapters covering the common phenomenology of movement disorders and includes tremor, dystonia, chorea, myoclonus, and tics. The etiology, pathophysiology, symptoms and treatments of these conditions are briefly reviewed, with the emphasis on the most common practical challenges encountered with these patients.Differential Diagnosis of Movement Disorders in Clinical Practice is aimed at postgraduates training in neurology, emergency care physicians, internists and primary care providers. Medical students will also find this book particularly helpful during their neurology rotations.  Monobina Gupta, "Didi - A Political Biography" English | 2012 | ISBN: 9350291681 | EPUB | pages: 232 | 0.9 mb A timely biography of the woman who defeated the longest-serving communist government in the world Mamata Banerjee is many things to many people - to some she is a 'performer' on the stage of Indian politics; to others she is a spirited woman who made it despite having no male patrons, a leader who was tested to the limit and emerged on top. If she is adored as the quintessential woman next door who has none of the airs that mark Indian politicians, she is as strongly derided for her 'theatricality' and rhetoric. But everyone agrees that she is a fighter who never gives up. Overcoming severe odds to challenge three decades of Marxist rule in West Bengal, Mamata Banerjee, 'Didi' to the young and old, came up trumps to become the first woman chief minister of West Bengal. But her real challenge begins now. Will she be able to end the culture of violence and regenerate West Bengal economically and politically? Will she be able to go beyond being a rabble-rouser to become a sagacious and visionary leader? Or will her party follow in the footsteps of the CPI(M), dragging the state deeper into the abyss it has fallen into? In Didi, Monobina Gupta brings her experience as journalist and commentator on politics in the state of West Bengal to paint a fascinating portrait of one of the most important political figures in India today.  Dictyostelium discoideum Protocols By Christina Schilde, Pauline Schaap (auth.), Ludwig Eichinger, Francisco Rivero (eds.) 2013 | 479 Pages | ISBN: 1627033017 | PDF | 11 MB Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of D. discoideum in recent years.The second edition of Dictyostelium discoideum Protocols incorporates the most recent developments in a number of fields. The book is divided into four parts. The first part provides an introduction to the amoebozoa and community resources. The second part presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence. The third part is dedicated to molecular genetics techniques, cell biological, biochemical and biophysical methods. The chapters in the fourth part describe the use of Dictyostelium as a model system to study vesicle formation, trafficking and infection by bacterial pathogens. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Dictyostelium discoideum Protocols, Second Edition seeks to aid scientists in the application of innovating techniques to study a range of fundamental biological processes in this attractive model organism.  Joe Bamford, "Devotion to a Calling: Far-East Flying and Survival with 62 Squadron RAF" English | ISBN: 1848841493 | 2010 | 160 pages | EPUB, MOBI | 14 MB + 18 MB Harley Boxall was awarded his RAF wings on 3 October 1936 and was posted to 40 Squadron flying Hawker Hinds. On 1 April 1937 he was posted to Bircham Newton in Norfolk to train with 206 Squadron which was a General Reconnaissance and Training unit equipped with the Avro Anson. He then joined 62 Squadron shortly after it was formed and received its first Blenheim in February 1938. During the summer of 1939 and because of increasing political tension in Europe, a decision was taken to reinforce the Far East Air Force with two squadrons of Blenheims. The urgency of the situation required that the aircraft, from 34 and 62 Squadrons, be flown out to their final destination at Tengah in Singapore. It took a total fifty flying hours to arrive  Developing B2B Social Communities: Keys to Growth, Innovation, and Customer Loyalty by Margaret Brooks English | PDF | 2013 | 196 Pages | ISBN : 1430247134 | 2.8 MB Developing B2B Social Communities: Keys to Growth, Innovation, and Customer Loyalty explains why business-to-business companies need a robust online community strategy to survive and flourish in today's changing economy and shows you how to design and execute your company's strategy successfully. |
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