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 Vascular Disease: Molecular Biology and Gene Therapy Protocols By Shu Ye, Adriano M. Henney (auth.), Andrew H. Baker (eds.) 1999 | 441 Pages | ISBN: 0896037312 | PDF | 3 MB In Vascular Disease: Molecular Biology and Gene Therapy Protocols, Andrew Baker and a noted panel of expert investigators describe today's most powerful molecular methods for investigating the pathogenesis of vascular disease. These detailed, easy-to-follow techniques range from methods that have been used successfully to identify specific mutations involved in cardiovascular disorders, to those for transferring genes associated with cardiovascular disease into various vascular cell types by in vitro and in vivo routes. There are methods to identify novel genes and generate full-length cDNAs, to study gene transcription and promoter activity easily and effectively, and to ascertain precisely gene expression levels within the individual cell types in different pathophysiological conditions. Accurate methods to quantify apoptosis in both cultured cells and pathological specimens are also given. Vascular Disease: Molecular Biology and Gene Therapy Protocols offers today's vascular biologist and gene therapist an unprecedented ability to study the pathogenesis of vascular disease and readily to probe the potential for gene-based therapies. Powerful and productive, the techniques presented here operate across a wide range of exciting research areas, and promise spectacular therapeutic breakthroughs in the ongoing battle against vascular disease.  Vaccine Protocols By Gordon Ada (auth.), Andrew Robinson, Michael J. Hudson, Martin P. Cranage (eds.) 2003 | 416 Pages | ISBN: 1588291405 | PDF | 3 MB This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development.  Vaccine Adjuvants: Preparation Methods and Research Protocols By Robert Edelman (auth.), Derek T. O'Hagan (eds.) 2000 | 342 Pages | ISBN: 0896037355 | PDF | 3 MB Adjuvants play a key role in controlling the efficacy of vaccines, through enhancing the immune response to vaccine antigens, including recombinant proteins, protein polysaccharide conjugates, synthetic peptides, or DNA. In Vaccine Adjuvants: Preparation Methods and Research Protocols, Derek T. O'Hagan and a team of expert vaccinologists and pharmacologists thoroughly describe the preparation, characterization, and evaluation of a wide range of alternative vaccine adjuvants for use in preclinical studies. Each chapter carefully reviews a single adjuvant, including suggestions why a specific adjuvant might be preferred for a given antigen, depending on what type of immune response is desired. Alternate adjuvant choices are also presented so that researchers can choose those most efficacious for their specific purpose. The methods, described in full detail, range from the simple mixing of an antigen with a preformed adjuvant, to a complex preparation process requiring the antigens to be physically associated, or entrapped within, an adjuvant formulation. Comprehensive and highly practical, Vaccine Adjuvants: Preparation Methods and Research Protocols provides an effective guide to making and using vaccine adjuvants. By closely following directions from the book, today's researchers will be able optimally to induce specific immune responses against different types of antigens and to selectively manipulate the immune response in a favorable way. The use of the information in this book will promote the development of powerful new vaccines against infectious diseases and cancers, and may promote the development of products to ameliorate autoimmune disorders.
 Unity Certified Programmer Exam Guide: Pass the Unity certification exam with the help of expert tips and techniques, 2nd Edition English | 2022 | ISBN: 1803246219 | 766 Pages | PDF EPUB (True) | 42 MB
 Emre Erşen, "Turkey's Pivot to Eurasia: Geopolitics and Foreign Policy in a Changing World Order " English | ISBN: 0367085666 | 2019 | 200 pages | EPUB | 2 MB This book discusses and analyses the dimensions of Turkey's strategic rapprochement with the Eurasian states and institutions since the deterioration of Ankara's relations with its traditional NATO allies.  Alexander D. Poularikas, "Transforms and Applications Primer for Engineers with Examples and MATLAB®" English | 2010 | pages: 570 | ISBN: 1138417947, 1420089315 | PDF | 8,6 mb Transforms and Applications Primer for Engineers with Examples and MATLAB® is required reading for engineering and science students, professionals, and anyone working on problems involving transforms. This invaluable primer contains the most essential integral transforms that both practicing engineers and students need to understand. It provides a large number of examples to explain the use of transforms in different areas, including circuit analysis, differential equations, signals and systems, and mechanical vibrations.  Transcription Factor Protocols By Dennis K. Watson, Richard Kitching, Calvin Vary (auth.), Martin J. Tymms (eds.) 2000 | 306 Pages | ISBN: 0896035735 | PDF | 2 MB In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results.Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today.  Benson, "Trans Love: an anthology of transgender and non-binary voices" English | 2019 | ISBN: 178592432X | EPUB | pages: 296 | 1.0 mb Selected as a 2019 LGBT Book of the Year by Dazed and Ms. Magazine
 Toward an International Virtual Observatory: Proceedings of the ESO/ESA/NASA/NSF Conference Held at Garching, Germany, 10-14 June 2002 By P. J. E. Peebles (auth.), Peter J. Quinn, Krzysztof M. Górski (eds.) 2004 | 344 Pages | ISBN: 3540210016 | PDF | 4 MB The book is the first thorough overview of the first important steps to develop a worldwide virtual observatory so that, in the future, it could be easier to "dial-up" a part of the sky than wait many months to access a telescope. The articles in this book present details on the status of the first efforts to develop a standardized framework for the virtual observatory, including steps towards completion and deployment of technical infrastructure, uptake by data providers worldwide, and utilization by the scientific community.  Nelson Armstrong , "The future fuel: A concise guide to the new hydrogen economy, renewable energy, sustainability, and creation of world energy power without pollution." English | 2022 | ASIN: B0B4GV1VRN, B0B3VHFZ7Q | EPUB | pages: 54 | 0.2 mb Hydrogen can be produced from almost all energy sources and hence has spite up interest with its demand rapidly increasing. The transformation of the energy will be fully based on the hydrogen economy . This book brings together discussions on energy, the future of hydrogen, splitting processes, the hydrogen rainbow, and a lot more with a focus on today's renewable energy sources. |
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