Created Anum Ahmad, PhD | Last updated 10/2021 Duration: 1h 28m | 9 sections | 22 lectures | Video: 1280x720, 44 KHz | 745 MB Genre: eLearning | Language: English + Sub
Learn about gene expression analysis techniques and their applications in diagnosis and treatment of cancer. What you'll learn Gene and its components What is gene expression? Introduction to gene expression techniques Techniques for mRNA expression analysis Introduction to hybridization based methods Northern blot RNA-FISH DNA microarray Introduction to PCR based methods RT-PCR Real time qRT-PCR Introduction to sequencing based techniques Sanger method High-throughput sequencing technology Serial Analysis of Gene Expression (SAGE) RNA sequencing Applications of gene expression analysis What is cancer? Oncogenes and tumor suppressor genes Challenges in understanding cancer genetics Techniques used to study cancer Targeted therapy for cancer Requirements Basic knowledge of biology and molecular biology Interested in learning latest technologies Description The field of molecular biology has a profound impact in life science investigation. The development of more and more sophisticated experimental techniques in molecular biology with a broad, interdisciplinary applicability have stimulated research and progress in almost all the disciplines of life science. The rationality and approach of scientific experimentations have changed and allowing revolutionizing discoveries not only in molecular biology but also in biochemistry, biophysics, biotechnology, cell biology, and genetics. Reserachers can choose suitable techniques based on their usefulness, objectives and the financial and temporal resources available to the them. Many molecular biology techniques have extensively been used in the study of gene expression. Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product (mRNA) that enables it to produce end products (protein), and ultimately affect a phenotype, as the final effect. The technique
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