Growth Factors and Receptors A Practical Approach

Growth Factors and Receptors: A Practical Approach by Ian A. McKay and Kenneth D. Brown
English | September 17, 1998 | ISBN-10: 019963646X | 288 pages | PDF | 13,5 MB
Growth Factors and Receptors: A Practical Approach provides comprehensive protocols for studies of growth factors and their interactions with receptors. It covers a wide range from simple analytical techniques to sophisticated in vivo applications including: RT-PCR and immunocytochemistry for detection of growth factors and receptors; production and purification of recombinant growth factors and receptors; labelling of growth factors for binding studies; in vivo mutagenesis; the yeast two-hybrid assay of protein-protein interactions; phage display of factors; application of factors to wound-healing processes using the gene gun; treatment of cancers with factor/toxin chimeras; and analysis of important factor domains using chimeric proteins.