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English | 2021 | ISBN: 0128220589 | 452 pages | True pdf, epub | 127.75 MB
Correlative Light and Electron Microscopy IV, Volume 162,a new volume in theMethods in Cell Biologyseries, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more.
Contains contributions from experts in the fieldCovers topics using nano-SIMS and EDX for CLEMPresents recent advances and currently applied correlative approachesGives detailed protocols, allowing for the application of workflows in one's own laboratory settingCovers CLEM approaches in the context of specific applicationsAims to stimulate the use of new combinations of imaging modalities
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