Human Naïve Pluripotent Stem Cells

Free Download Peter Rugg-Gunn, "Human Naïve Pluripotent Stem Cells "
English | ISBN: 1071619071 | 2022 | 298 pages | EPUB | 40 MB
This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.