Published 7/2022 MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHz Language: English | Size: 895.94 MB | Duration: 2h 50m sterilization and culture media concepts
What you'll learn Introduction sterilization Definition and Methods Physical Methods - Red Heat Hot Air Oven Moist heat and auto clave Pasteurization IR radiation Non IR Filtration 5 types of Filtration chemical method- two types of gases Liquid stage 4 types of Liquid chemicals Applications Introduction Culture methods culture media definition and ingredients types of media physical media semi solid media solid media chemical media 3 types non synthetic media special media 3 types Enriched media selective media differential media anaerobic media Transport media Antibiotic media conclusion Requirements All life science students Description Introduction sterilizationDefinition and MethodsPhysical Methods - Red HeatHot Air OvenMoist heat and auto clavePasteurizationIR radiationNon IRFiltration5 types of Filtrationchemical method- two types of gasesLiquid stage4 types of Liquid chemicalsApplicationsIntroduction Culture methodsculture media definition and ingredientstypes of mediaphysical mediasemi solid mediasolid mediachemical media 3 typesnon synthetic mediaspecial media 3 typesEnriched mediaselective mediadifferential mediaanaerobic mediaTransport mediaAntibiotic mediaconclusionTwo major contributions to the art of sterilization came in the 1860'swhen the French chemist and microbiologist Louis Pasteurwrote extensively on how germs cause disease and the English physician, Joseph Lister,developed a technique that used carbolic acid as a spray to disinfect instruments and so on. (refer video slides all full info)The first liquid artificial culture medium was created by Louis Pasteur in 1860Previously bacterial growth on daily materials such as some foods had been observedLouis Pasteur was a French chemist and microbiologist renowned for his discoveries of the principles of vaccination microbial fermentation and pasteurizationThe German physician Robert Koch (December 11 1843 — May 27 1910) is considered the father of modern bacteriology for his work demonstrating that specific microbes are responsible for causing specific diseasesKoch discovered the life cycle of the bacteria responsible for anthrax and identified the bacteria that cause tuberculosis and choleraCulture mediaA microbiological culture medium is a substance that encourages the growth support and survival of microorganisms Culture media contains nutrients growth promoting factors energy sources buffer salts minerals metals and gelling agents (for solid media)Different types of culture media are typically divided based on the physical state of the media Liquid culture media commonly called broth Solid and semi-solid culture media commonly called agar. (refer video slides all full info) Overview Section 1: Introduction Lecture 1 Introduction Lecture 2 DEFINITION AND METHODS1 Lecture 3 Red heat flaming, Physical method continues Lecture 4 Hot air oven Lecture 5 Moist heat or auto clave Lecture 6 Disinfectant types or Pasteurization explanation Lecture 7 Radiation Lecture 8 NONIR RADIATION Lecture 9 FILTRATION Lecture 10 CHEMICAL METHOD Lecture 11 CHEMICAL METHOD CONTINUEOUS Lecture 12 APPLICATIONS Lecture 13 Culture Methods Intro Lecture 14 Culture Media Lecture 15 Types Media Part1 Lecture 16 Types Media Part2 Lecture 17 Types of Media Part3 Lecture 18 Types of Media Part4 Lecture 19 Part 4 Continues Non synthetic Media Lecture 20 Types of Media Part5 Lecture 21 Types of Media Part 6 Lecture 22 Types of Media Part7 Lecture 23 Types of Media Part8 Lecture 24 Types of Media Part9 Lecture 25 Types of Media Part10 Lecture 26 Conclusion All life science students
