Sterilization And Culture Methods Detailed

What you'll learn
Introduction sterilization
Definition and Methods
Physical Methods - Red Heat
Hot Air Oven
Moist heat and auto clave
Pasteurization
IR radiation
Non IR
Filtration
5 types of Filtration
chemical method- two types of gases
Liquid stage
4 types of Liquid chemicals
Applications
Introduction Culture methods
culture media definition and ingredients
types of media
physical media
semi solid media
solid media
chemical media 3 types
non synthetic media
special media 3 types
Enriched media
selective media
differential media
anaerobic media
Transport media
Antibiotic media
conclusion
Requirements
All life science students
Description
Introduction sterilizationDefinition and MethodsPhysical Methods - Red HeatHot Air OvenMoist heat and auto clavePasteurizationIR radiationNon IRFiltration5 types of Filtrationchemical method- two types of gasesLiquid stage4 types of Liquid chemicalsApplicationsIntroduction Culture methodsculture media definition and ingredientstypes of mediaphysical mediasemi solid mediasolid mediachemical media 3 typesnon synthetic mediaspecial media 3 typesEnriched mediaselective mediadifferential mediaanaerobic mediaTransport mediaAntibiotic mediaconclusionTwo major contributions to the art of sterilization came in the 1860'swhen the French chemist and microbiologist Louis Pasteurwrote extensively on how germs cause disease and the English physician, Joseph Lister,developed a technique that used carbolic acid as a spray to disinfect instruments and so on. (refer video slides all full info)The first liquid artificial culture medium was created by Louis Pasteur in 1860Previously bacterial growth on daily materials such as some foods had been observedLouis Pasteur was a French chemist and microbiologist renowned for his discoveries of the principles of vaccination microbial fermentation and pasteurizationThe German physician Robert Koch (December 11 1843 — May 27 1910) is considered the father of modern bacteriology for his work demonstrating that specific microbes are responsible for causing specific diseasesKoch discovered the life cycle of the bacteria responsible for anthrax and identified the bacteria that cause tuberculosis and choleraCulture mediaA microbiological culture medium is a substance that encourages the growth support and survival of microorganisms Culture media contains nutrients growth promoting factors energy sources buffer salts minerals metals and gelling agents (for solid media)Different types of culture media are typically divided based on the physical state of the media Liquid culture media commonly called broth Solid and semi-solid culture media commonly called agar. (refer video slides all full info)
Overview
Section 1: Introduction
Lecture 1 Introduction
Lecture 2 DEFINITION AND METHODS1
Lecture 3 Red heat flaming, Physical method continues
Lecture 4 Hot air oven
Lecture 5 Moist heat or auto clave
Lecture 6 Disinfectant types or Pasteurization explanation
Lecture 7 Radiation
Lecture 8 NONIR RADIATION
Lecture 9 FILTRATION
Lecture 10 CHEMICAL METHOD
Lecture 11 CHEMICAL METHOD CONTINUEOUS
Lecture 12 APPLICATIONS
Lecture 13 Culture Methods Intro
Lecture 14 Culture Media
Lecture 15 Types Media Part1
Lecture 16 Types Media Part2
Lecture 17 Types of Media Part3
Lecture 18 Types of Media Part4
Lecture 19 Part 4 Continues Non synthetic Media
Lecture 20 Types of Media Part5
Lecture 21 Types of Media Part 6
Lecture 22 Types of Media Part7
Lecture 23 Types of Media Part8
Lecture 24 Types of Media Part9
Lecture 25 Types of Media Part10
Lecture 26 Conclusion
All life science students

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